Drug concentration was increased at every passage at a rate of 0.2nM till 1µM resistance was achieved and designated as resistant K562 cell line (K562R). ©2009-2021 Elabscience, Inc. All Rights Reserved. Growth Medium for K562 RPMI1640 10% FBS Anti-Anti; 5 mL per 500 mL of culture medium Procedure A. Freeze medium: 60% Basal medium+30% FBS+10% DMSO. Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells. ATCC human aml cell lines k562 Human Aml Cell Lines K562, supplied by ATCC, used in various techniques. The parental K562 cell line was transduced with a lentivi-ral vector expressing CD64 or CD32, and the cells were sorted and cultured as described in Materials and Methods. (B) Number of Hi-C loops in K562 cells that overlapped with top 613 interactions called by three methods from POLR2A ChIA-PET data in K562 cells. Methods in Molecular Biology, vol 1441. Call us Toll Free at 1-800-457-3801. Studies conducted by Anderson, et al. The data support the conclusion that K-562 is, indeed, a multipotential leukemia cell line of human origin. (C) Fraction of interactions called by three methods from POLR2A ChIA-PET data in K562 cells that are validated by 5C interactions in K562 cells. CD32 in K562 cells. 59: 77-82, 1977. Recommendations for handling of cryopreserved cells. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. One tube containing 100 g is provided at a concentration of 1 mg/mL. J. Natl. Your email subscription to News & Announcements has been received. The parental K562 cell line was transduced with a lentivi-ral vector expressing CD64 or CD32, and the cells were sorted and cultured as described in Materials and Methods. K562 lacks the B markers of immunoglobulin, Epstein‐Barr virus (EBV) genome and associated nuclear antigen, and receptors for EBV. The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. possible, and a separate shipping charge will be included for each shipping method required. All trademarks are the property of their respective owners. 2) When ready to start cell culture, quickly thaw ampoule in a 37oC water bath. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Population highly undifferentiated and of the granulocytic series. Leuk. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4. By signing up, you indicate that you have read and agree to the terms in our privacy policy. Even if imatinib is used as a tyrosine kinase inhibitor (TKI) for CML therapy, drug resistance ma … K562 lacks the B markers of immunoglobulin, Epstein‐Barr virus (EBV) genome and associated nuclear antigen, and receptors for EBV. Search results for k562 at Sigma-Aldrich. Elabscience provides you with stable K-562 [K562] Cell Line to meet your cell-related research needs. If you're having trouble with stimulating the NK cells you may be culturing the K562 cell too long. Note: not available in United States, please don't order online. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. Current Position:Home>>Cell Products>>Cell Line>> K-562 [K562] Cell Line. The National Centre for Cell Science (NCCS), an autonomous organisation aided by the Department of Biotechnology, Government of India, was established to facilitate cell biology research in the country. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. Electroporate 1 million K562 cells with 2 μg of gRNA plasmid, 2 μg of wild-type Cas9 expression vector (Addgene #41815), and 4 μg of donor using the Amaxa 4D-Nucleofector (Lonza) per manufacturer's recommendations. With a wealth of cell-based immortalization experience, AcceGen scientists have been able to successfully immortalize a variety of mammalian cells using different immortalization methods. In this chapter, a robust NK cell expansion protocol is described using K562 cell line gene modified to express membrane bound IL21 (K562 mb.IL21). Choose a Store Santa cruz biotechnology. Cultures can be maintained by the addition of fresh medium or replacement of medium. bvseo-msg: The resource to the URL or file is currently unavailable.. As expected, FAK was not activated in K562 IM-S cells compared with K562 IM-R Adh cells treated with imatinib (Figure 6A). PubMed: 163658. The cells carry the NPM-ALK fusion gene. NCCS is located within the academically-enriched campus of the Savitribai Phule Pune University (SPPU). (b) Relative binding of anti-CD3 to CD32- and CD64-transduced K562 cells. K562 Cell Line: The K-562 cell line was originally established from the bone marrow of a 53-year-old female patient who suffered from chronic myelogenous leukemia (CML). Somanchi S.S., Lee D.A. Add 4, 8, 12, 16, and 20 μL soup into each well of sample set, and supplement volume to 200 μL with medium. Blood 45: 321-334, 1975. 1- Control with 4 μg of preimmune Rabbit IgG; 2- … S1 blocked K562cells in the G1 phase of cell cycle .S1 showed a synergistic effect when combined with Cytosine Arabinoside Hydrochloride (Ara-c) in inducing K562 cell apoptosis. K562 cells (ATCC CCL-243) are maintained at 37°C under 5% CO 2 in RPMI medium supplemented with 10% FBS, penicillin–streptomycin, and GlutaMAX.. 2. Take K562 cells of logarithmic growth phase to be inoculated in three 96-well plates with density at 1 × 10 5 /cm 2, each well with 100 μL. PubMed: 95026. Res. The strain obtained by the ATCC most closely resembles the B population. Karyological studies on various K-562 sublines have been classified into three groups(A,B,C) by Dimery, et al. 89121407). Lozzio CB, Lozzio BB. Shipping charges for countries outside the US and Canada will be determined once order has been received, Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. *Please select more than one item to compare CML cells were maintained in expo-nential growth phase and resistance developed by treating the cells with gradual stepwise increasing concentrations of imatinib starting from 2nM. IP samples: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate. Items are grouped and shipped together whenever The K562 cell line derived from a CML patient in blast crisis was examined for properties of B and T lymphocytes and cell lines. 118: 355-361, 1977. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available. health products, bulky and/or heavy items. Data are shown as mean ± s.d.. Data of major editing events, ranging from +1 NHEJ to –30 MMEJ, were compiled from editing outcomes of 30 gRNAs targeting different sites at … Chronic myeloid leukemia (CML) is a hematopoietic malignancy characterized by the t(9; 22) and the related oncogene, BCR-ABL. Compare Products: Select up to 4 products. Alaska, Hawaii, Canada and Puerto Rico. Myeloperoxidase activity was found in 5 to 10% of the cells. (b) Relative binding of anti-CD3 to CD32- and CD64-transduced K562 cells… CD32 and CD64 aAPCs were Application Natural killer assay and tumourigenicity studies K562 cells were incubated with viral supernatants for 24 h, ... Buy from Supplier : human aml cell lines k562 91. Antibody Sampler Kit for studying Trail-R4/DcR2 in the Apoptosis research area. The results indicate that a precise orchestration of signals, including ERK1/2 and p38 MAPKs as well as PI3K pathway, is necessary for acquisition of features of mature MKs. The line is used as an in vitro target for the natural killer assay. Bioz Stars score: 91/100, based on 1 PubMed citations. K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. Polyploid cells (5-8%) and early myelomonocytic precursors have PAS-positive material, were stained with Sudan black, and possessed abundant acid phosphatase. Growth Medium for K562 RPMI1640 10% FBS Anti-Anti; 5 mL per 500 mL of culture medium Procedure A. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. https://www.elabscience.com/p-k_562_k562_cell_line-58778.html (2016) Ex Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21. In this case you should purchase a new vial from ATCC. Mitochondrial Membrane Potential Assay Kit (with JC-1), Cell Proliferation and Toxicity Detection Kits, Cell Viability/Proliferation/Cytotoxicity, 200mM L-Alanyl-L-Glutamine Solution (100×). This method is used for less temperature sensitive items such as lab ware and animal To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. Santa Cruz Animal Health. 1. This is a clone of the parental K562 cell line which is resistant to the tyrosine kinase inhibitor imatinib mesylate, and is paired to the K562-s clone which is sensitive to imatinib. K562 cells (ATCC CCL-243) are maintained at 37°C under 5% CO 2 in RPMI medium supplemented with 10% FBS, penicillin–streptomycin, and GlutaMAX.. 2. Create your Question below, or click "Reply" to Answer others' questions. In vitro antitumor activity of some 4-(2-hydroxyaryl)-1,2,3-thidiazoles was studied against erythroleukemia (K562) and cervical carcinoma (HeLa) cell lines. The original Jurkat clone was designated JM*. J. Immunol. Bioz Stars score: 99/100, based on 1715 PubMed citations. Lead Time: Order now, ship in 3 daysWelcome to order from local distributors. The present work with K562 cells provides new insights into the molecular mechanisms regulating MK differentiation. Andersson LC, et al. Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. The cells that were removed from the patient are tumorigenic, and exhibit lymphoblast morphology. Shipping charges listed below are from our US warehouses to the Contiguous US, The target cells (K562) are stained with 5 µM calcein AM for 30 min; The target cells were then seeded into the 96-well plate at 10,000 cells/well; Next, the effector cells were added following the E:T ratios shown on the plate map (below) Finally, IL-2 was added to half of the wells to activate NK cells K-562 cells have been characterized as being largely undifferentiated. AcceGen has developed the proprietary Cell Immortalization Service for research application. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. Receipt of Frozen Cells and Starting Cell Culture 1) Immediately place frozen cells in liquid nitrogen freezer storage until ready to culture. To place an order using RMB or to ship to mainland China, please visit www.scbio.cn, Click on image or enlarge button to enlarge, PROTEIN A, G-PLUS & L AGAROSE IP REAGENTS, NUCLEOTIDES, PEPTIDES, PROTEINS & AMINO ACIDS, AUTORADIOGRAPHY FILM, TAPE AND PLASTIC WRAP, whole cell lysate provided as Western blotting positive control, loc_en_US, sid_2203, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)], 500 µg protein in 200 µl SDS-PAGE Western blotting buffer, human whole cell lysate; chronic myelogenous leukemia cells, should be stored at -20°C and repeated freezing and thawing should be minimized, sample vial should be boiled once prior to use. Cancer Inst. This cell line is derived from a patient with anaplastic large cell lymphoma, ALK positive. The line is EBNA negative. , on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. Copyright © 2007-2021 Santa Cruz Biotechnology, Inc. All Rights Reserved. KARPAS 299 cell line which carries the NPM-ALK fusion gene. In: Somanchi S. (eds) Natural Killer Cells. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. West WH, et al. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH Lozzio BB, Lozzio CB. Background and Research Application The KARPAS 299 cell line is a Human Non-Hodgkin's Ki-positive Large Cell Lymphoma cell line useful in oncology research. Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. Choose your product and application below, then click "Create my review".Then complete and submit your review on the next page. You have the right to unsubscribe at any time by clicking the 'unsubscribe' link in any News & Announcement emails you receive. K-562 (human chronic myelogenous leukemia) Whole Cell Lysate is provided as a Western blotting positive control. ... K562 cells were treated with CDDO-Me at 0.25, 0.5, and 1 μM for 24 h. A. Maintaining healthy cells is the key to experimental success and reproducible research results. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA c Results: K562 cells were exposed to various concentrations of S1 for 6h, MTT experiments showed that S1 inhibit proliferation on dose-dependent manner. Electroporate 1 million K562 cells with 2 μg of gRNA plasmid, 2 μg of wild-type Cas9 expression vector (Addgene #41815), and 4 μg of donor using the Amaxa 4D-Nucleofector (Lonza) per manufacturer's … 1. PubMed: 69036. Can be used as a highly sensitive target for the in vitro natural killer assay. Millipore k562 cells K562 Cells, supplied by Millipore, used in various techniques. The highest-quality RNA available from Ambion, it is DNase-treated and subjected to unsurpassed quality control standards. We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Buy from Supplier : k562 cells 99. Representative blots of Bip, p-PERK, PERK, p-IRE1α, IRE1α, CHOP, and β-actin. 3: 363-370, 1979. Cells were sorted by puromycin followed by quantitative PCR and western blots with indicated antibodies. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc. Receipt of Frozen Cells and Starting Cell Culture 1) Immediately place frozen cells in liquid nitrogen freezer storage until ready to culture. The same patterns were observed in iPSCs, U937 cells, K562 cells and primary T cells. Ortaldo JR, et al. CD32 in K562 cells. The cell population has been characterized as highly undifferentiated and of the granulocytic series. Research using hematopoietic cells and other suspension cells has been limited in part by low efficiency of gene transfer (transduction and transfection) into these cell types. Failure of ITGβ3 engagement, by seeding cells on uncoated plates or by using a blocking antibody, impaired imatinib-induced FAK/Akt phosphorylation and constitutive ERK1/2 activation and resensitized IM-R Adh cells to imatinib-induced caspase 3 activation (Figure 6 B). The role of protein adsorption in the forced adhesive growth of K562 leukemic cells onto a cationic surface composed of polylysine was investigated. The K562 cell line derived from a CML patient in blast crisis was examined for properties of B and T lymphocytes and cell lines. Cell Line Origin Human Caucasian chronic myelogenous leukaemia Cell Line Description A subclone of the parent line K562 (Sigma Catalogue number. Subscribe to get resources and special offers. 2) When ready to start cell culture, quickly thaw ampoule in a … Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Representative ~0.5-Mb genomic region showing SuRE signals of HG02601, SuRE library 1 in K562 cells, together with DNase-seq and H3K27ac signals in K562 cells … Yes, in nude mice. Tyrosine kinase activity of fusion protein BCR-ABL is the main cause of CML.

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